Fig. 6

Baicalin and wogonoside altered the phosphorylation and expression of AMPK, PI3K, and AKT in HG-induced human podocyte cells. a Human podocyte cells were preconditioned with HG (30 mmol/L) for 0, 5, 10, 15, and 30 min and measured by western blotting. b Human podocyte cells were preconditioned with HG (30 mmol/L) for 0, 0.5, 1, 2, and 3 h, and measured by western blotting. c Human podocyte cells were preconditioned with baicalin (5 or10 mg/L) or wogonoside (5 or10 mg/L) for 12 h and incubated for 0.5 h with different treatments. The expression of AKT and p-AKT was measured by western blotting. d Human podocyte cells were preconditioned with baicalin (5 or10 mg/L) or wogonoside (5 or10 mg/L) for 12 h and incubated for 2 h with different treatments. The expression of AMPK, p-AMPK, PI3K, and p-PI3K was measured by western blotting. Control: normal glucose condition (5 mM D-glucose); DMSO:dimethyl sulfoxide; HG: high glucose condition (30 mM D-glucose); MA (mannitol, 5 mM D-glucose + 25 mM mannitol)