Fig. 4

Treatment of JIMT-1 cells with different solvent fractions of Ethiopian plants inhibits TNF-⍺-induced translocation of p65/NF-κB to the nucleus. a p65/NF-κB positive nuclei in % of total nuclei number. The data are presented as mean ± SD with n = 3. *p < 0.05; **p < 0.001. b Representative images from independent experiments for each treatment. Control, no treatment. TNF-α, only treated with TNF-α. SO, aqueous fraction of S. oxyacanthum (52 μg/mL) and TNF-α. CS, chloroform fraction of C. simensis (80 μg/mL) and TNF-α. VL, ethyl acetate fraction of V. leopoldi (0.87 μg/mL) and TNF-α. To visualize the p65/NF-κB expression (green), the cells were fixed in formaldehyde and labeled as described in the Methods section. All images were taken with a 40x objective