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Table 5 Minimal inhibitory concentrations of tested samples in presence of PAβN

From: Bark extract of Cassia sieberiana DC. (Caesalpiniaceae) displayed good antibacterial activity against MDR gram-negative phenotypes in the presence of phenylalanine-arginine β-naphthylamide

Bacterial strains Tested samples and concentrations (μg/mL)
CSB CSBc CSBc1 CSBc2 CSBc3 Chloramphenicol
MIC +PAβN R MIC +PAβN R MIC +PAβN R MIC +PAβN R MIC +PAβN R MIC +PAβN R
Escherichia coli
 ATCC8739 512 64 8 512 16 32 256 16 16 256 64 4 256 256 1 2 < 0,5 > 4
 AG102 512 64 8 512 32 16 nd 256 < 4 > 64 64 > 8 32 4 8
Klebsiella pneumoniae
 ATCC11296 512 < 1 512 128 4 64 16 4 256 128 2 < 4 > 128 32 8 4
 KP55 128 < 4 > 32 128 256 0.5 nd 256 > 2 nd 64 32 2
Enterobacter aerogenes
 ATCC13048 512 16 32 512 256 2 512 < 1 256 32 8 < 4 > 128 16 32 2
 EA27 32 > 16 < 4 128 64 > 8 256 > 2 nd 32 16 2
Providencia stuartii
 ATCC29916 512 32 16 512 256 2 nd 256 128 2 256 8 32 64 8 8
 PS2636 512 32 16 512 128 4 nd 256 < 2 nd 64 16 4
Pseudomonas aeruginosa
 PA01 512 nd 512 < 1 nd nd < 4 > 128 64 8 8
 PA124 512 nd 512 256 2 nd 128 256 0,5 16 > 32 256 16 16
PIA (%)   70    80    30    70    60    100  
  1. MIC minimal inhibitory concentration R = AIF: MICample alone / MICample+PAβN ratio (this means the factor which determine the improvement of the activity of samples by PAβN; the activity of a sample was considered to be improved when its AIF was > 2). (+ PAβN): represent MIC values of tested samples obtained in presence of PAβN (−): MIC > 512 μg/mL for crude extract, fractions and sub-fractions PIA: percentage of improved activity nd: not determined (as no MIC values were not observed till 512 μg/mL) CSB: crude extract CSBc: fraction CSBc1, CSBc2 and CSBc3 are sub-fractions from fraction CSBc