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Table 1 Quantitative analysis of the viability of the cultured RGC-5 cell line utilizing MTT assay

From: Emodin protected against retinal ischemia insulted neurons through the downregulation of protein overexpression of β-catenin and vascular endothelium factor

Group (n = 5 ~ 6) Control Pre-OGD vehicle Pre-OGD Emo 0.25 μM Pre-OGD Emo 0.5 μM
Cell viability (% of control) 100 38.30 ± 2.51%*** 43.81 ± 3.75% 47.52 ± 3.99%†
  1. The value in each group was the ratio of the viability of the cultured RGC-5 cells relative to that of the control group, which was set as 100%. This study include 4 groups including control (normal; RGC-5 cells that were incubated in culture medium containing vehicle), OGD (1 h of pre-OGD administration of vehicle before the OGD condition), Pre-OGD 0.25 μM (1 h pre-OGD administration of 0.25 μM emodin before the OGD condition), and Pre-OGD 0.5 μM (1 h pre-OGD administration of 0.5 μM Emodin). *** denotes an extremely significant difference (P < 0.001) between the normal control and the OGD group. † denotes a significant difference (P = 0.04) between the OGD group and the pre-OGD application of emodin 0.5 μM group. The results are mean ± SE (n = 5 ~ 6). Abbreviations: RGC-5 retinal ganglion cell-5, MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, OGD oxygen glucose deprivation, Emo Emodin