Fig. 1

The viability of the cultured RGC-5 cell line was quantitatively analysed using MTT assay. The value in each group was the ratio of the viability of the cultured RGC-5 cells relative to that of the control group, which was set as 100%. This study includes four groups, namely control (normal; RGC-5 cells which were cultured in culture medium containing vehicle), OGD (1 h of pre-OGD treatment with vehicle), Pre-OGD 0.25 μM (1 h of pre-OGD treatment with 0.25 μM emodin), and Pre-OGD 0.5 μM (1 h of pre-OGD treatment with 0.5 μM Emodin). *** identifies that the normal control significantly differs (P < 0.001) from the OGD group. † designates a significant difference (p = 0.04) between the OGD group and the pre-OGD application of emodin 0.5 μM group. The results are mean ± SE (n = 5 ~ 6). Abbreviations: RGC-5, retinal ganglion cell-5; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; OGD, oxygen glucose deprivation; Emo, Emodin