Skip to main content
Fig. 3 | BMC Complementary Medicine and Therapies

Fig. 3

From: Jianpi Yangwei decoction promotes apoptosis and suppresses proliferation of 5-fluorouracil resistant gastric cancer cells in vitro and in vivo

Fig. 3

BGC823/5-Fu establishment. a. BGC823 cells were oval with clear boundary, uniform size, uniform cytoplasm and few irregular cells. BGC823/5-Fu cells were fusiform and more irregular in shape. (Magnification 400×); b. MTT assay showed that, with the increase of 5-Fu concentration, the inhibition rate of sensitive cells and drug-resistant cells both increased, but the inhibition rate of drug-resistant cells was lower than sensitive cells at the same concentration; c. The IC50 of 5-Fu on BGC823/5-Fu cells was 26.180 μg/ml, while on BGC823 was 2.012 μg/ml, thus, the drug RI of BGC823/5-Fu was 13 compared with BGC823; d. The real-time RT-PCR detection showed that the mRNA expression of MDR1 in BGC823/5-Fu cells (0.001291563 ± 0.000262) was significantly higher than BGC823 cells (0.000544291 ± 0.000123) (P < 0.01)

Back to article page