Fig. 8

Confocal microscopy images of acridine orange/ethidium bromide double stained DU-145 cells reveals necrotic pathway in the presence of EAB and annonacin treatment. Treatment with (b) 50 μg/mL EAB extract and (c) 0.6 μg/mL annonacin for 72 h compared to (a) untreated cells as control reveal necrotic cells (red in appearance, red arrow) in annonacin and EAB treatment groups, in comparison to viable healthy cells as stained uniformly green (blue arrow) in (A) in the control group. Magnification 10 ×