Fig. 5

EAB extract and annonacin does not alter the mitochondrial membrane potential (MMP) in DU-145 cells. Quantitative evaluation of the effects of annonacin and EAB extract on MMP in DU-145 cells after 72 h was carried out using JC-10 Fluorescence dye computed relative to untreated cells (control). Cell were treated with 10 μM H₂O₂ and 2 nM docetaxel as positive control as docetaxel disrupts MMP. EAB extract and annonacin displayed healthy cell content of near 100% similar to the control, while those treated with docetaxel yielded 69% healthy cell and hydrogen peroxide displayed 39% healthy cell ratio, as measured by the healthy (red) cell count in comparison to the damaged (green) cells. . Data are means ± SEM of triplicates in three independent experiments. Statistical significance indicated by *** (one-way ANOVA, P < 0.0001)