Fig. 2

a-d. Dose dependent effect of TC on mRNA expression pattern of apoptotic markers Bax (2A), Caspase-3 (2B), HIF-α (2C) and Bcl2 (2D) in control and experimental groups. β-actin mRNA was used as housekeeping gene for the normalization of mRNA expressions. Quantification graphs values are expressed as mean ± SEM. ## indicates p < 0.01 vs normal control group; *and **- indicates p < 0.05 and 0.01, respectively, vs ISO group. Mean difference between the groups was analyzed using one-way ANOVA, followed by Tukey’s multiple comparison as post-hoc test. A p value < 0.05 was considered as statistically significant