Fig. 2

Effects of PL treatment and upregulation of miR-34b-3p on the proliferation, invasion, and apoptosis of NSCLC cells in vitro. a Relative expression of miR-34b-3p in normal human bronchial epithelial (NHBE) cells and lentivirus-infected A549 and H1299 cells using qPCR. b, c Optical density value was detected to represent the cell proliferation ability by CCK-8 Kit in the different groups. d, e Cell apoptosis was determined by flow cytometry and the caspase3/7 activity assay. f Cell invasion ability was detected 24 h after PL treatment by the transwell assay. A549 and H1299 were infected with miR-34b-3p lentivirus or treated with PL, then transwell assay was performed to detect invasive cell number. Invasive cells were stained with crystal violet and cell images were captured, then cell number were counted. All of the experiments were repeated three times