Fig. 5

Flow cytometric analysis of Annexin V staining of MDA-MB-231 cells treated with AELE. Topotecan was used as a positive control. A significant increase in Annexin-V binding was reported after 24 h of treatment with increasing AELE concentrations. Dunnett’s test was used to compare the different concentrations to the control. Two-way ANOVA was used to determine the significant differences reported with * indicating a p-value: 0.01 < p < 0.05, ** indicating a p- value: 0.001 < p < 0.01, and *** indicating a p-value: p < 0.001