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Fig. 9 | BMC Complementary Medicine and Therapies

Fig. 9

From: An insight into anti-adipogenic properties of an Oroxylum indicum (L.) Kurz extract

Fig. 9

The effect of OIE on the ATP level at day 12. The level of ATP was determined by ATP bioluminescent. On day 12, the cells were incubated with an ATP releasing agent and transfer to the 96 well plates. The luciferase assay was added to the sample, then the amount of light emission was measured using a microplate luminometer. ATP concentration was determined from the standard curve. The protein concentrations in each group were used to normalize the ATP content (ηM/mg of protein). Means ± SD value (n = 9) of three independent experiments is indicated. ND: non-differentiated cells (pre-adipocytes); ND + OIE(200): non-differentiated cells with OIE at 200 μg/mL (OIE-treated pre-adipocytes); D: differentiated cells with 0.1% DMSO (untreated-adipocytes); D + OIE(200): differentiated cells with OIE at 200 μg/mL (OIE-treated adipocytes). Differences among groups were determined by one-way ANOVA followed by Tukey’s Post-hoc test; the different superscript alphabets are significantly different from each other (P < 0.05)

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