Fig. 3

The effect of the OIE on the expression of Cdk2 in 3 T3-L1 cells. a Western blot analysis of the expression of Cdk2 in cells treated with OIE at a concentration range from 50 μg/mL to 200 μg/mL at 24 h. The total lysates of 3 T3-L1 cells with the treatment mentioned above were resolved on Mini-PROTEAN TGX gels followed by transfer to PVDF membrane and probed with the anti-Cdk2 antibody (primary antibody) and secondary antibody. The protein was visualized using chemidoc with the ECL detection kit. b The densitometry analysis of Western blot bands was normalized against β-actin and expressed as a mean of ± SD of three independent experiments (n = 9). ND: non-differentiated cells (pre-adipocytes); D: differentiated cells with 0.1% DMSO (untreated-adipocytes); D + OIE(200): differentiated cells with OIE at 200 μg/mL (OIE-treated adipocytes). Differences among groups were determined by one-way ANOVA followed by Tukey’s Post-hoc test, and the different superscript alphabets are significantly different from each other (P < 0.05). The full-length of the blots are presented in Supplementary Figure 1