Fig. 11
From: An insight into anti-adipogenic properties of an Oroxylum indicum (L.) Kurz extract
The effect of OIE on mitochondria morphology. On day 12, the cells were extracted, then fixed in 2.5% glutaraldehyde, and post-fixated with 1% Osmium tetroxide. The fixed sample was embedded in epoxy resin. The sample was sectioned at 60 nm with the ultra-microtome. The sections were stained with 2% uranyl acetate, followed by secondary staining with lead stain solution. The grids were observed under a TEM at 120 kV. The micrographs of mitochondria in 3 T3-L1 cells showed at scale bar 500 nm and 200 nm. Two independent experiments (each run triplicate) were operated. ND: non-differentiated cells (pre-adipocytes); ND + OIE(200): non-differentiated cells with OIE at 200 μg/mL (OIE-treated pre-adipocytes); D: differentiated cells with 0.1% DMSO (untreated-adipocytes); D + OIE (200): differentiated cells with OIE at 200 μg/mL (OIE-treated adipocytes)