Table 1 Primer sequence for genes
Name | GenBank accession no. | Forward primer(5′-3′) | Reverse primer(5′-3′) | Amplicon length |
|---|---|---|---|---|
IL-1β | NM214055 | GAAAGATAACACGCCCACC | GGAGTTTCCCAGGAAGACG | 193 |
IL-6 | M80258 | CTTCAGTCCAGTCGCCTTCT | GCCAGTACCTCCTTGCTGTTT | 234 |
IL-10 | L20001 | GACCAGATGGGCGACTTGTT | CACGGCCTTGCTCTTGTTTT | 243 |
IL-12p35 | NM213993 | ATGACAACCCTGTGCCTTAG | TTCAGAGCCTGCATCAGC | 158 |
IFN-γ | AY188090 | TTCAAAGGAGCATGGATGTG | CTCTTCCGCTTTCTTAGGTTAG | 196 |
TNF-α | JF831365 | CCCCCAGAAGGAAGAGTTTC | GGCATTGGCATACCCACTCT | 160 |
PRL-19 | AF435591 | AACTCCCGTCAGCAGATCC | AGTACCCTTCCGCTTACCG | 147 |