Fig. 7From: Fermented mulberry (Morus alba) leaves suppress high fat diet-induced hepatic steatosis through amelioration of the inflammatory response and autophagy pathwayTranscription of autophagy related genes in liver tissue. The levels of Atg4b, Atg5, Atg7, and Atg12 mRNA in the total transcripts of liver tissue were measured by qRT-PCR analyses using their specific primers. The relative levels of the Atg4b, Atg5, Atg7, and Atg12 mRNA were calculated based on the intensity of β-actin as an endogenous control. Three to five mice per group were used in the preparation of the total RNAs, and qRT-PCR analyses was assayed in duplicate for each sample. The values of data represent the mean ± SD. Asterisk symbol (*) indicates significant P < 0.05 compared to the No treated group. Sharp symbol (#) indicates significant P < 0.05 compared to the HFD + Vehicle treated groupBack to article page