Fig. 2

Infiltration of mast cells and expression levels of inflammatory mediators. a The infiltrated mast cells in the tissue sections of liver collected from HFD + EMfC treated mice were stained with toluidine blue, followed by observed and counted at 400x magnification. The arrow head in histological image indicates mast cells infiltrated into the liver tissue. Three to five mice per group were used for the preparation of the tissue sections, and toluidine blue staining and cell counting was analyzed in duplicate in each liver tissue. b The levels of iNOS and COX-2 mRNA in the total transcripts of liver were measured by qRT-PCR analyses using their specific primers. The relative levels of the iNOS and COX-2 mRNA were calculated based on the intensity of β-actin as an endogenous control. Three to five mice per group were used in the preparation of the total RNAs, and qRT-PCR analyses was assayed in duplicate for each sample. The values of data represent the mean ± SD. Asterisk symbol (*) indicates significant P < 0.05 compared to the No treated group. Sharp symbol (#) indicates significant P < 0.05 compared to the HFD + Vehicle treated group