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Table 1 Chemical constituents identified in the cell extract

From: NMR and LCMS analytical platforms exhibited the nephroprotective effect of Clinacanthus nutans in cisplatin-induced nephrotoxicity in the in vitro condition

Peak

Metabolites

δH ppm (multiplicity)

1

Isoleucine

0.95 (d, 7.4 Hz), 1.00 (d, 7.0 Hz)

2

Leucine

0.96 (d, 6.5 Hz)

3

Valine

0.99 (d, 6.8 Hz), 1.05 (d, 6.8 Hz)

4

Ethanol

1.35 (d)

5

Threonine

1.33(d, 6.5 Hz), 4.24 (m)

6

Lactate

1.33(d, 6.7 Hz), 4.11 (m)

7

Alanine

1.48 (d, 7.2 Hz)

8

Acetate

1.95 (s)

9

Glutamate

2.06 (m), 2.12 (m), 2.35 (m)

10

Glutathione

2.17 (m), 3.78 (m)

11

Aspartate

4.59 (d, 8.0 Hz), 2.83 (m)

12

Lysine

4.73 (d, 8.0 Hz), 4.10 (m), 2.94 (s)

13

Phosphocholine

4.74 (m), 3.47 (s)

14

Choline

3.24 (s)

15

Glycerophosphocholine

4.74 (m), 3.47 (s)

16

Scyllo-inositol

3.34 (s)

17

Betaine

3.89 (s)

18

Creatine

3.04 (s), 3.94 (s)

19

Myo-inositol

4.06 (t,10 Hz), 3.55 (dd, 2.9, 3.0 Hz), 3.63 (t, 9.5 Hz)

20

Dimethylamine

2.73 (s)

21

Formate

8.45 (s)

22

Glucose-1-phosphate

5.67d

23

ATP,ADP,AMP

7.84 (m), 8.63(m)

24

Glutamine

2.14 (m), 3.77 (m)

25

Pyruvate

2.35 (s)

26

Glucose

5.22 (d, 3.8 Hz)

27

Glycine

3.56 (s)

28

Tyrosine

3.42 (t, 5.1 Hz)

29

Histidine

6.98 (m)

30

Phenylalanine

7.32 (m), 7.37 (m)

31

Tryptophan

7.67 (m)

  1. J coupling values (in Hz). Multiplicity: singlet (s), doublet (d), triplet (t), doublet of doublet (dd), multiplet (m)