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Fig. 3 | BMC Complementary Medicine and Therapies

Fig. 3

From: NMR and LCMS analytical platforms exhibited the nephroprotective effect of Clinacanthus nutans in cisplatin-induced nephrotoxicity in the in vitro condition

Fig. 3

a MTT assay on NRK-52E after pre-treated with different solvent extracts of C. nutans at 1000 μg/ml for 24 h followed by 48 h treatment with cisplatin at 20 μM. b LDH analysis on NRK-52E after pre-treated with different C. nutans extracts at 1000 μg/ml for 24 h followed by 48 h induction with cisplatin at 20 μM. (20–20% ethanol extract; 40–40% ethanol extract; 60–60% ethanol extract; 80–80% ethanol extract; EtOH-100% ethanol extract and cis-20 μM cisplatin). The relative LDH release (%) related to control wells containing cell culture medium without any treatment. *Statistically significant difference compared to control (p < 0.05). ** Statistically significant difference compared with cisplatin (p < 0.05)

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