Fig. 6

Inhibition of osteoclastogenesis in bone marrow progenitor cells and the effect of GS on cathepsin K expression of osteoclasts. a Bone marrow stem cells were treated at 1, 5 and 10 μM concentrations of GS for 9 days in differentiation medium for osteoclast formation. 1 nM of E2 was used as a positive control. Images were captured under a phase-contrast microscope. Scale bar = 0.1 mm (b) The number of TRAP-positive multinucleated cells were scored and represented as % TRAP-positive cells. Scale bar = 0.1 mm. c Absolute qPCR analysis for cathepsin K mRNAs expression (d) Immunofluorescence stain of cathepsin K. Values are expressed as mean ± SEM (n = 3); ^*P < 0.05 compared with control