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Fig. 5 | BMC Complementary Medicine and Therapies

Fig. 5

From: Genistein contributes to cell cycle progression and regulates oxidative stress in primary culture of osteoblasts along with osteoclasts attenuation

Fig. 5

Effect of GS on mRNA levels of osteogenic genes and immunolocalization of the BMP2 protein in osteoblasts. Rat calvarial osteoblasts were treated with different concentrations 1, 5 and 10 μM of GS for 48 h. Absolute qPCR was performed to analyze the mRNA expressions of (a) Runx2 (b) BMP2 and (c) Osteocalcin genes in osteoblasts. d Photomicrographs show the immunofluorescence staining of BMP2 under inverted fluorescence microscope; magnification = 20x and scale bar = 0.1 mm. Values were obtained from three independent experiments performed in the triplicate set and expressed as mean ± SEM; *P < 0.05

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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