Fig. 1

Microscopic observation and percent cell proliferation of primary osteoblasts. (a) Chemical structure of Genistein (b) Morphology of osteoblasts under inverted phase-contrast microscope treated with different concentrations 1, 5 and 10 μM of GS at 48 h. Scale bars = 0.1 mm (c) Percent cell proliferation of osteoblasts treated with different concentrations of GS. Cells treated with 1 nM of E2 was used as a positive control. Values were obtained from three independent experiments and expressed as mean ± SEM. *P < 0.05 as compared with control