Fig. 6

Effects of 5FEM on cell cycle of A549-Homo BIRC5 cells. The cells were treated with different 5FEM concentrations for 48 h and subjected to flow cytometry. The representative DNA fluorescence histogram of PI-stained A549-Homo BIRC5 cells showing the cell cycle distribution; a 2.5 μM 5FEM, b 5.0 μM 5FEM. c 10.0 μM 5FEM. d 10.0 nM YM155. e Vehicle (0.1% DMSO) control. f RPMI 1640 medium control. g Column figure of cell cycle rate in different groups. All data were expressed as mean ± SE of three experiments, and each experiment included triplicate repeats. *p < 0.05, ***p < 0.001 vs. vehicle control