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Table 1 Morphological effects of the methanol extract from the of leaves and twigs of B. macowanii on B. cereus

From: Disruption of microbial cell morphology by Buxus macowanii

Morphological changes

SEM

TEM

Control

Buxus macowanii

Control

Buxus macowanii

Cell shape

Uniformly rod-shaped

swollen cells (CWS)

Uniformly rod-shaped

Cells are swollen and contracted

Cell surface

Smooth cell surfaces

rough cell surface (RC)

Smooth cells surfaces

Rough cell surface (RC)

Cytoplasmic membrane

Intact

aMembrane damage (DWC) and loss of intracellular contents (LCC)

Cytoplasm was homogenously electron dense

Partial loss of cytoplasmic electron density

Cell wall

Intact

Damaged cell wall (DWC) (Perforations)

Intact

Cell wall distortion

Cell division

Complete

Incomplete cell division (ICD)

Complete

Incomplete cell division (ICD)

Cell viability

Viable

Cell death

Viable

Cell death

  1. aNo DNA extravasion carried out. However, the combination of the damage to the cell wall, perforations and other changes observed point to cytoplasmic membrane damage and loss of intracellular contents