Fig. 2

PAMs reduced cancer cell growth in human glioma TMZ-sensitive (U251S), TMZ-resistant (U251R) and mouse GL-26 cells as determined by colony formation assay. a PAMs reduced cell growth as determined by colony formation in glioma cell lines (U251S, U251R and GL-26). 500 cells were seeded in each well, treated with PAMs for 48 h, incubated for additional 8–10 days, cells were fed every two days. b Cells were treated by PAMs (50 μg/ml), TMZ (15 μM), or PAMs (50 μg/ml) + TMZ (15 μM). Combination of PAMs with low dose TMZ significantly inhibited cell growth as shown by colony formation assay. Data represent the mean ± SEM. The p-value was calculated by t-test. *p < 0.05, **p < 0.01, compared to untreated control (0 μg/ml). The untreated control group was taken as 100%. After combined treatment of PAMs and TMZ, percentage of colonies were 30, 60, and 60 (colony size> 100 mm) in U251S, U251R, and GL-26 cells respectively. Experiments were performed in triplicate and repeated three times with similar results (n = 3)