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Fig. 9 | BMC Complementary Medicine and Therapies

Fig. 9

From: Haimufang decoction, a Chinese medicine formula for lung cancer, arrests cell cycle, stimulates apoptosis in NCI-H1975 cells, and induces M1 polarization in RAW 264.7 macrophage cells

Fig. 9

The activation and polarization of RAW264.7 cells in the presence of and absence of HMF. RAW264.7 cells were treated with LPS (1 μg/mL) or IL-4 (10 ng/mL) or different concentrations (15.6–125 μg/mL) of HMF for 24 h, and then incubated with the indicated antibodies. The expression of macrophage activation marker-cluster of differentiation (CD) 40 (a), M1 phenotype signature marker CD 86 (b), and M2 phenotype signature marker CD 206 (d) were detected by Aria FACS flow cytometry system (Beckman Coulter MoFlo XDP, Fullerton, CA, USA). Immunofluorescence staining of CD86 (red) (c) on macrophages and the nuclei stained with DAPI (blue) were imaged using a confocal laser scanning microscope (CLSM, Olympus, Tokyo, Japan) (magnification × 200). Quantitative analysis of the relative median fluorescence intensity of CD86 by Image-Pro Plus software. Different colors were used to distinguish the peaks corresponding to different groups. Each experiment was performed in triplicate, and values were represented as means ± SD. * P < 0.05, and ** P < 0.01 versus the control

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