Fig. 5

Effect of HMF on the expression of apoptosis-associated protein in NCI-H1975 cells. a The expression levels of apoptosis-associated proteins in NCI-H1975 cells treated with HMF (0–250 μg/mL) for 48 h were analyzed by western blotting using anti-P53, anti-BCL-2 associated X protein (BAX), anti-B-cell lymphoma-2 (BCL-2), anti-cleaved-cysteinyl aspartate specific proteinase-3 (C-Cas3), anti-cleaved-cysteinyl aspartate specific proteinase (C-Cas9), anti-cleaved-poly-adenosine diphosphate-ribose polymerase (C-PARP). b The results of the western blot analysis were quantified; n = 3. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01 vs. control