Fig. 4

ATEO regulates the expression of neuroprotective proteins in C6 cells. In neurotrophic (NGF, BDNF and GDNF, a) and anti-oxidation (GPx1, SOD2 and UCP2, b) activity, cultured C6 cells were treated with ATEO (15 μg/mL) for 48 h. In anti-inflammation (IL-1β, IL-6 and TNF-α, c) activity, cells were treated with ATEO (15 μg/mL) for 48 h before TNF-α and IFN-γ (10 + 10 ng/mL) co-treatment for another 24 h. Cells were collected to determine the mRNA amount. Forskolin (FSK, 10 μM) and ZLN005 (2 μM) were used as positive control. Data are expressed as percentage of control (untreated culture), and in mean ± SEM, where n = 3. *p < 0.05, **p < 0.01, ***p < 0.001 compared with control