Table 1 Primer sequences of miRNA-204 and target genes
miRNA-204 | RT | 5′-GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAG CCAACAGGCAT-3’ | [23] |
Forward | 5′-GCGGCGGTTCCCTTTGTCATCCT-3’ | ||
Reverse | 5′- GTGCAGGGTCCGAGGT-3’ | ||
PARP1 | Forward | 5′-GACGTGGAGAGCATGAAGAAGG-3’ | (Designated) |
Reverse | 5′-GGTGTAGAAGCGATTGGAGAGA-3’ | ||
HIF1α | Forward | 5′-CAAAGACAATAGCTTTGCAGAATG-3’ | [24] |
Reverse | 5′-ACGGTCACCTGGTTGCTG-3’ | ||
NFATc2 | Forward | 5′-GGCAGCAGATTTGGGAGATGG-3’ | (Designated) |
Reverse | 5′-GTGCTGTGGGTAATATGGCTGG-3’ | ||
a-SMA | Forward | 5′-CCTGGCTTCGCTGTCTACCT-3’ | [25] |
Reverse | 5′-TTGCGGTGGACGATGGA-3’ | ||
RNU6 | Forward | 5′-CTCGCTTCGGCAGCACA-3’ | [26] |
Reverse | 5′-AACGCTTCACGAATTTGCGT-3’ | ||
18 s | Forward | 5′-AGTCCCTGCCCTTTGTACACA-3’ | [27] |
Reverse | 5′-GATCCGAGGGCCTCACTAAAC-3’ |