Fig. 5

Curcumin inhibited CCL3 production of OCPs in the trabecular bone of OVX mice. OVX or sham-operated 10-weeks-old WT mice were fed 4 g of the control diet or the diet containing 200 mg/kg curcumin each day (for 4 weeks; n = 6 ~ 10, per group). (a) The typical femoral 3D MicroCT reconstructed images in each group. Scale bar, 100 μm. (b) The typical TRAP-stained femoral sections in each group (red arrowheads). Scale bar, 5 μm. (c) Femoral sections were stained with green and red fluorochromes for RANK and CCL3, respectively, and observed under fluorescent microscopy. The overlaps of RANK and CCL3 are indicated by white arrows (yellow fluorescence). Scale bar, 2.5 μm. Data are representative images among 6 independent samples with unanimous results. (d-g) The trabecular bone parameters (BMD, BV/TV, Tb.N and Tb.Sp) were analysed by using Micro-CT. (h) The calculation regarding osteoclasts number per millimeter of trabecular bone surface. (i) Serum levels of CCL3 were measured using ELISA kits. Data are expressed as the mean ± SEM. *P < 0.05. CUR, curcumin