Fig. 4

CCR1 overexpression did not promote the osteoclastogenesis in the presence of curcumin. (a) The representative TRAP staining images of osteoclasts derived from viruses-transduced OCPs under the combined intervention of M-CSF, RANKL and 25 μM curcumin or PBS for 5 days. Scale bar, 200 μm. (b-d) The histogram shows the quantitative results regarding mature osteoclasts (more than three nuclei), large osteoclasts (more than five nuclei) or osteoclast size (the ratio of large osteoclasts/total osteoclasts) in A. Data are expressed as mean ± SEM from three independent experiments, *P < 0.05 by Student’s t-test; ns, no significance. CUR, curcumin; M, M-CSF; R, RANKL