Fig. 5

Cytotoxicity of the most frequently used plants by the THs for the treatment of helminthiasis: Human PBMCs (2 × 10⁵ cells/well) were left alone (Med) or stimulated with 200 μg/ml of AM, KS, XA, DMSO 100 and 10% for 24 h. Cells were stained with propidium iodide dye (PI) and acquired by flow cytometry. a: lymphocytes gate, b, c, and d are PI⁺ cells in presence of AM, KS and XA respectively. (E) Box whiskers (tukey) with outliers show the percentage of lymphocytes expressing PI (n = 13). P values were determined by Mann-Whitney U-test. The NF EN ISO 10993-5 standards classifications were indicated by class I (not cytotoxic), class II (moderate cytotoxicity), class III (Benign cytotoxicity) and class IV (severe cytotoxicity)