Evaluation of in-vivo antidiarrhoeal and in-vitro antibacterial activities of the root extract of Brucea antidysenterica J. F. Mill (Simaroubaceae)

Table 5 Zone of inhibition for the root extract of B. antidysenterica against selected diarrhoea causing bacteria

Extract	Concentration	S. flexneri (ATCC-12022) (mm)	S. typhi (ATCC-13062) (mm)	Salmonella Spp. (CI) (mm)	E. coli (ATCC-25922) (mm)	E. coli (CI) (mm)	P. aeruginosa (ATCC-27853) (mm)
80% BAE	BAE 200 mg/mL	14.7 ± 0.33 ^a3d2	14.7 ± 0.33 ^a3c2d3	NA	14.0 ± 0.00 ^a3c2d3	NA	16.7 ± 0.33 ^a3c3d3
	BAE 400 mg/mL	17.3 ± 0.67 ^a3d1	17.7 ± 0.33 ^a3b2	NA	16.7 ± 0.33 ^a3b1d2	NA	19.7 ± 0.33 ^a3b3d2
	BAE 800 mg/mL	20.7 ± 0.33 ^a3b2c1	19.3 ± 0.33 ^a3b3	NA	18.3 ± 0.33 ^a3b1c3	NA	22.0 ± 0.00 ^a3b3c2
Standards	Cipro 5 μg/disc	29.3 ± 1.20	34.3 ± 0.67	18.0 ± 0.00	32.3 ± 0.33	NA	–
Standards	Cefta 30 μg/disc	–	–	–	–	–	10.3 ± 0.33

Data are expressed as Mean ± SEM (n = 3), ^a compared to positive control; ^b compared to 200 mg/mL; ^c compared to 400 mg/mL; ^d compared to 800 mg/mL; ¹P < 0.05, ²P <0.01, ³P <0.001. The negative control has shown no antibacterial activity. The positive controls: BAE Brucea antidysenterica extract, Cipro Ciprofloxacin, Cefta Ceftazidime, NA No Activity

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