Table 2 Primers used for RT-PCR
Gene | Primer sequence |
|---|---|
IL-4 | Forward: 5′-ACAGGAGAAGGGACGCCAT-3′ |
Reverse: 5′-GAAGCCCTACAGACGAGCTCA-3′ | |
IFN-γ | Forward: 5′- TGAGCTCATTGAATGCTTGG −3′ |
Reverse: 5′- GGCCATCAGCAACAACATAA −3′ | |
TNF-α | Forward: 5′-GGAAAGGACGGACTGGTGTA-3′ |
Reverse: 5′- TGCCACTGGTCTGTAATCCA −3′ | |
GAPDH | Forward: 5′-TGGTGAAGGTCGGTGTGAAC-3′ |
Reverse: 5′-ACTGTGCCGTTGAATTTGCC-3′ |