Fig. 3

Effects of ASP on mitochondrial apoptosis in LSK cells. a: Apoptosis was assessed in different groups. b: The ROS levels were assessed by inverted fluorescence microscopy. c: The MMP levels were tested by using flow cytometry. d: Outer membrane integrity was assessed. E: Flow cytometry scatter plot of apoptosis. f: Immunofluorescence images of ROS. G: Flow cytometry scatter plot of MMP. h, i: Markers of mitochondrial apoptosis and the p38/MAPK signaling pathway were tested by Western blotting. The results for the treated group indicate that the medium-dose ASP treatment reduces mitochondrial apoptosis in BMNC cells. Note: ▲P < 0.05 and ▲▲P < 0.01 compared with the normal control group. *P < 0.05 and **P < 0.01 compared with the model control group; #P < 0.05 and ##P < 0.01 compared with the low-dose group; ■P < 0.05 compared with the medium-dose group