Table 1 UPLC conditions for melittin analysis
From: The antibacterial activity and toxin production control of bee venom in mouse MRSA pneumonia model
Item | Condition | ||
|---|---|---|---|
Column | Halo ES-C18(2.1 × 100 mm, 2.7 μm, Advanced materials technology, USA) | ||
Flow rate | 0.8 mL/min | ||
Column temperature | 50 °C | ||
Injection volume | 2 μl | ||
Wavelength | 220 nm | ||
Mobile phase | Time (min) | 20 mM TFAa/MeCN(%) | 20 mM TFA/H2O(%) |
0 | 40 | 60 | |
6 | 45 | 55 | |