Fig. 1

The comparison of DNA repair ability of BGC823 and BGC823/5-Fu. a Procedure of LP-BER assay in cells. A DNA oligo containing the damaged DNA lesion 8-oxo-dG was transfected into BGC823 and BGC823/5-Fu cells; 2 h later, cells were lysed, and released 8-oxo-dG was determined by ELISA. b Relative repair capacity of two cells. c Reconstitution of long-patch (LP) base excision repair (BER) with cell lysate . The top part showed the schematic structure of the corresponding DNA substrates. The middle showed PAGE-separated products and the bottom showed the relative percentage of repaired product at different cell extract concentrations