Fig. 3

Salsola komarovii ethanol extract (SEE) does not induce glucocorticoid response element (GRE)-driven adipogenesis. a Effect of SEE on GRE induction, determined using the luciferase reporter gene assay. HepG2 cells were transiently transfected with the Cignal GRE reporter reagent including the GRE-containing reporter and Renilla plasmid. The transfection process and data analysis were similar to those of the NF-κB-driven luciferase reporter gene assay in Fig. 2b. The HC-treated group was used as the positive control. ***p < 0.001 vs. untreated group. b and c Effect of SEE on lipid accumulation in 3 T3-L1 cells. b The preadipocytes were differentiated in the absence or presence of the SEE or HC for 8 days, and then stained. Oil Red O-stained cells were photographed and the representative microscopic images of three independent experiments are presented. Original magnification 200×; scale bar = 200 μm. c Lipid content in Oil Red O-stained adipocytes was quantified using the absorbance measured at 520 nm. The results are presented as mean ± standard error of the mean (SEM) of three independent experiments. ***p < 0.001 vs. untreated group