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Fig. 4 | BMC Complementary Medicine and Therapies

Fig. 4

From: Endothelium-dependent and endothelium-independent vasorelaxant effects of unripe Rubus coreanus Miq. and Dendropanax morbiferus H. Lév. extracts on rat aortic rings

Fig. 4

Effects of DP, Hy-DP and 5-uRCK on the expression of NOS isoforms at the mRNA level (as assessed by RT-PCR). a RT-PCR analysis of iNOS mRNA expression in murine RAW 264.7 macrophages. Activated cultures were stimulated for 6 h with 1 μg/mL LPS or individual extracts before extraction of RNA. b RT-PCR analysis of nNOS mRNA expression in cultured rat hippocampal neurons. Activated cultures were stimulated for 6 h with 50 μM L-glutamate (Glu) or individual extracts before extraction of RNA. c RT-PCR analysis of eNOS mRNA expression in HUVECs. Activated cultures were stimulated for 6 h with 10 μM ACh or individual extracts before extraction of RNA. d eNOS mRNA expression was detected by real-time quantitative RT-PCR. *P < 0.05, **P < 0.01 and ***P < 0.001 vs the CTL group; NS, not significant

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