Fig. 4

Betulinic acid promotes PANC-1 and SW1990 cells apoptosis. Flow cytometry for apoptosis [apoptosis ratio was calculated as (Q2 + Q3)/(Q1 + Q2 + Q3 + Q4)] of PANC-1 and SW1990 cells incubated with Betulinic acid (20 μM, 60 μM) or an equal volume of DMEM medium for 24 h.. *Data are presented as mean ± SD, N = 3; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, compared with control