Preclinical anticancer studies on the ethyl acetate leaf extracts of Datura stramonium and Datura inoxia

Table 4 Cytotoxicity and protein kinase inhibition of DSL-EA and DIL-EA extracts

Samples	Brine shrimp cytotoxicity		Protein kinase inhibition			Cytotoxicity
	Brine shrimp cytotoxicity		Protein kinase inhibition			PC-3		MDA-MB 231		MCF-7		Normal lymphocytes
	% Mortality 200 μg/ml	LC₅₀ μg/ml	Diameter at 100 μg/disc		MIC μg/disc	% inhibition 20 μg/ml	IC₅₀ μg/ml	% Inhibition 20 μg/ml	IC₅₀ μg/ml	% Inhibition 20 μg/ml	IC₅₀ μg/ml	% Inhibition 20 μg/ml	IC₅₀ μg/ml
	% Mortality 200 μg/ml	LC₅₀ μg/ml	Clear zone	Bald zone	MIC μg/disc	% inhibition 20 μg/ml	IC₅₀ μg/ml	% Inhibition 20 μg/ml	IC₅₀ μg/ml	% Inhibition 20 μg/ml	IC₅₀ μg/ml	% Inhibition 20 μg/ml	IC₅₀ μg/ml
DSL-EA	96.96 ± 5.25^a	12.04 ± 0.21	7.5 ± 0.58	12.50 ± 0.58^c	100	14.4 ± 0.4^b	–	38.03 ± 0.38^b	–	40.2 ± 0.3^b	–	4 ± 0.35^c	–
DIL-EA	100 ± 0.00^a	10.37 ± 0.56	9.00 ± 1	19.00 ± 0.58^b	25	99.93 ± 0.11^a	2.86 ± 0.1	98.64 ± 0.33^a	1.56 ± 0.16	99.36 ± 0.72^a	2.45 ± 0.04	23 ± 1.45^b	–
Vincristine	–	–	–	–	–	–	–	–	–	–	–	79 ± 2.55^a	6.89 ± 0.19
Doxorubicin	100 ± 0.00^a	5.74 ± 0.18	–	–	–	99.43 ± 0.15^a	2.95 ± 0.11	98.11 ± 0.48^a	2.45 ± 0.24	98.62 ± 0.42^a	3.2 ± 0.11	–	–
Surfactin	–	–	–	26.50 ± 1.0^a	–	–	–	–	–	–	–	–	–
DMSO	–	–	–	–	–	–	–	–	–	–	–	–	–

Values are presented as mean ± SD of triplicate analysis. Results of standards and/or controls are given in respective columns. Means with different superscripts (a-c) represent significantly different values at p < 0.05

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