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Table 10 Percent alleviative effects of DSL-EA and DIL-EA extracts in benzene induced leukemia

From: Preclinical anticancer studies on the ethyl acetate leaf extracts of Datura stramonium and Datura inoxia

Evaluation parameters Study Groups
Disease control Positive control DSL-LD (P) DSL-HD (P) DIL LD (P) DIL HD (P) DSL HD (T) DIL HD (T)
Percent alleviative effect
Haematalogical parameters
 RBCs (×106)/μl 4.33 ± 0.065 41.80 ± 2.20a 32.90 ± 1.65c 38.71 ± 1.92ab 35.64 ± 0.85bc 39.66 ± 1.51ab 23.79 ± 1.62d 32.74 ± 1.88c
 WBCs (×103) /μl 7.78 ± 0.012 50.53 ± 0.87b 53.54 ± 0.33a 52.14 ± %0.49a 50.39 ± 0.35b 48.46 ± 0.15c 45.67 ± 0.57d 45.28 ± 0.46d
 Platelets (×103)/μl 344 ± 3.19 44.08 ± 0.79e 56.42 ± 0.58b 61.81 ± 0.75a 52.37 ± 0.89c 58.15 ± 0.76b 49.76 ± 0.58d 45.25 ± 0.86e
Endogenous antioxidant enzymes
 CAT (U/min) 0.5 ± 0.04 342.67 ± 8.33b 84.67 ± 15.53d 100 ± 1.20d 274 ± 12.49c 285.3 ± 27.15c 315.3 ± 17.47bc 410 ± 30.27a
 POD (U/min) 1 ± 0.04 209 ± 6.56b 70.33 ± 2.52e 122 ± 1.73d 63.33 ± 4.16e 317.67 ± 3.21a 183.67 ± 9.07c 301 ± 10.15a
 SOD (U/mg protein) 0.96 ± 0.05 261.81 ± 3.66a 154.17 ± 2.08e 198.96 ± 4.17c 190.63 ± 2.08cd 232.29 ± 3.76b 176.7 ± 10.43d 191.6 ± 10.26cd
 GST (nM/min/ml) 81.01 ± 0.44 191 ± 1.81b 33.72 ± 0.78e 99.22 ± 0.87c 34.43 ± 1.28e 266.29 ± 1.98a 67.07 ± 0.78d 101.08 ± 1.91c
Biochemical parameters
 ALT (U/L) 93.20 ± 2.64 50.67 ± 2.01b 50.83 ± 2.45b 54.36 ± 1.92ab 52.47 ± 1.78ab 56.96 ± 2.43a 58.13 ± 2.10a 57.46 ± 1.70a
 AST (U/L) 67.80 ± 2.05 59.73 ± 0.94bc 65.23 ± 1.50a 61.91 ± 1.27ab 54.72 ± 1.89de 58.58 ± 1.39bcd 56.96 ± 1.01cde 53.79 ± 1.69e
 ALP (U/L) 356 ± 5.69 60.69 ± 1.01bcd 58.38 ± 0.92de 61.14 ± 1.14abc 57.28 ± 0.81e 59.43 ± 0.35cde 62.15 ± 1.01ab 63.38 ± 1.13a
 Urea (mg/dl) 72.2 ± 2.76 62.59 ± 1.41a 51.01 ± 1.37c 57.02 ± 2.44ab 50.15 ± 1.18c 52.29 ± 1.71bc 61.52 ± 2.84a 59.65 ± 2.60a
 Creatinine (mg/dl) 2.89 ± 0.45 61.01 ± 5.04a 60.21 ± 3.34a 61.82 ± 4.19a 60.32 ± 3.89a 62.40 ± 2.78a 61.25 ± 3.51a 61.94 ± 2.50a
 Bilirubin (mg/ml) 7.56 ± 0.97 65.21 ± 2.82a 58.38 ± 1.23a 63.10 ± 8.54a 59.79 ± 4.37a 60.76 ± 3.44a 68.17 ± 1.07a 68.08 ± 5.16a
 Total proteins (g/dl) 2.14 ± 0.11 214.02 ± 18.19a 185.51 ± 23.36a 224.45 ± 27.63a 213.55 ± 29.55a 210.28 ± 23.22a 186.29 ± 17.50a 195.02 ± 19.61a
Lipid peroxidation assay
 TBARs (nM/min/mg protein) 133.7 ± 2.61 35.74 ± 0.82a 15.08 ± 1.65d 24.27 ± 1.11c 15.12 ± 1.74d 21.39 ± 1.22c 37.41 ± 0.89a 31.01 ± 1.32b
  1. Results of disease control are expressed as mean ± SD (n = 6, where n = number of rats analysed in each group). Alleviative effects of remaining groups are expressed as percent increase or decrease (bold) of tested parameters in comparison to the disease control group. Groups are compared statistically to the positive control group and superscripts (a-e) in individual rows specifying significance at p < 0.05