Fig. 5

Modified PD mitigated inflammation in DSS-induced UC in vivo. Colon tissues of negative control group, model group (DSS-treated) and modified PD group (DSS-treated with modified PD treatment) were collected. (A) qRT-PCR results indicating modified PD treatment reduced mRNA level of IL-6, VEGF and TNF-α (Modified PD represent high dose modified PD treatment mice herein). (B) Effect of modified PD on the protein expression of NLRP3, STAT3, p-STAT3, NF-κB, p-NF-κB, p-p65, IL-1β, IL-6 and TNF-α. Protein expression levels were analyzed by Western Blot. (C) The quantitative analysis for western blot. *: p < 0.05, **: p < 0.01, ***: p < 0.001. Data are expressed as mean ± SEM.