Table 2 Analytical characteristics of the method validation

Precision and accuracy

1

Intra-day RSD (%), n = 5

0.437

0.357

0.487

0.67

0.56

0.45

2

Inter-day RSD (%),n = 5 (day-1/day-2/day-3)

0.437/0.495/0.512

0.353/0.425/0.532

0.484/0.498/0.514

0.67/0.68/0.71

0.56/0.59/0.63

0.45/0.48/0.49

Recovery

3

Amount of standard in MC samples (μg mg⁻¹) containing highest bioactive compounds

44.6

8.1

19.2

20.1

10.3

6.1

4

Amount of standards added in MC sample (μg mg^− 1)

20/40/60

4/8/12

10/20/30

10/20/30

5/10/15

3/6/9

5

Amount of standard found (μg mg⁻¹)

64.36/84.4/104.26

12.1/16.2/20.11

29.21/39.19/49.12

30.12/40.13/50.03

15.29/20.28/25.33

9.1/12.21/15.09

6

Recovery (%)

99.62/99.76/99.67 (99.81)

100/100.62/100.04

100.03/99.97/99.83

100.06/100.07/99.86

99.93/100.89/99.9/100.11

100/100.9/99.93