Skip to main content

Table 2 Analytical characteristics of the method validation

From: Validation and quantification of major biomarkers in ‘Mahasudarshan Churna’- an ayurvedic polyherbal formulation through high-performance thin-layer chromatography

Sr. no.ParametersMangiferin (M)Ursolic acid (UA)Oleanolic acid (OA)Gallic acid (GA)Quercetin (Q)Curcumin (C)
 Precision and accuracy      
1Intra-day RSD (%), n = 50.4370.3570.4870.670.560.45
2Inter-day RSD (%),n = 5 (day-1/day-2/day-3)0.437/0.495/0.5120.353/0.425/0.5320.484/0.498/0.5140.67/0.68/0.710.56/0.59/0.630.45/0.48/0.49
 Recovery      
3Amount of standard in MC samples (μg mg−1) containing highest bioactive compounds44.68.119.220.110.36.1
4Amount of standards added in MC sample (μg mg− 1)20/40/604/8/1210/20/3010/20/305/10/153/6/9
5Amount of standard found (μg mg−1)64.36/84.4/104.2612.1/16.2/20.1129.21/39.19/49.1230.12/40.13/50.0315.29/20.28/25.339.1/12.21/15.09
6Recovery (%)99.62/99.76/99.67 (99.81)100/100.62/100.04100.03/99.97/99.83100.06/100.07/99.8699.93/100.89/99.9/100.11100/100.9/99.93