Table 1 Method validation parameters for the quantification of Mangiferin, Oleanolic acid, Ursolic acid, Gallic acid, Curcumin and Quercetin

1

Linearity range (ng/spot; n = 12^a

200–800

200–3000

200–3000

500–2000

300–1500

100–1000

2

Correlation coefficient (r²)

0.987

0.991

0.997

0.988

0.992

0.99

3

Regression equation

Y = − 608.4 + 9.033X

Y = − 126.87.0 + 11.05X

Y = − 1111.13 + 10.0914X

Y = 1939 + 7.19X

Y = −200 + 1.74X

Y = 137 + 9.66X

4

Calculated SD value (CATS software)

6.72

2.59

3.26

5.33

2.12

2.43

5

^bLimit of detection (LOD) (ng) [3 x SD/S]

25

30

27

40

45

30

6

^bLimit of quantitation (LOQ) (ng) [10 x SD/S]

75

90

81

125

140

90

7

R_f

0.53

0.38

0.59

0.28

0.48

0.56

8

λmax

254 nm

9

Mobile phase

Ethyl acetate: glacial acetic acid: formic acid: water (20: 2.2: 2.2: 5.2 v/v)