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Table 1 Method validation parameters for the quantification of Mangiferin, Oleanolic acid, Ursolic acid, Gallic acid, Curcumin and Quercetin

From: Validation and quantification of major biomarkers in ‘Mahasudarshan Churna’- an ayurvedic polyherbal formulation through high-performance thin-layer chromatography

Sr.no.ParametersMangiferin (M)Ursolic acid (UA)Oleanolic acid (OA)Gallic acid (GA)Quercetin(Q)Curcumin(C)
1Linearity range (ng/spot; n = 12a200–800200–3000200–3000500–2000300–1500100–1000
2Correlation coefficient (r2)0.9870.9910.9970.9880.9920.99
3Regression equationY = − 608.4 + 9.033XY = − 126.87.0 + 11.05XY = − 1111.13 + 10.0914XY = 1939 + 7.19XY = −200 + 1.74XY = 137 + 9.66X
4Calculated SD value (CATS software)6.722.593.265.332.122.43
5bLimit of detection (LOD) (ng) [3 x SD/S]253027404530
6bLimit of quantitation (LOQ) (ng) [10 x SD/S]75908112514090
7Rf0.530.380.590.280.480.56
8λmax254 nm520 nm366 nm
9Mobile phaseEthyl acetate: glacial acetic acid: formic acid: water (20: 2.2: 2.2: 5.2 v/v)n-hexane: ethyl acetate: acetone (16.4: 3.6: 0.2 v/v)Toluene: ethyl acetate: formic acid (13.5: 9: 0.6 v/v)
  1. a Four concentration levels in triplicate; bSD is the standard deviation of the blank response and S is the slope of the calibration plot