Fig. 4

Different concentrations of ox-LDL induced the increase of inflammation and autophagy protein expression in HUVECs. a Protein expressions of LC3II, p62, LOX-1, VCAM1, MCP-1, β-actin in HUVECs. The cells were treated with 0 mg/L、20 mg/L、40 mg/L、60 mg/L、80 mg/L ox-LDL respectively for 24 h. b, c, d, e, f Bar charts show the mean intensity of every protein quantified and normalized versus β-actin expression. Values are submitted as mean ± S.D. based on seperate experiments in triplicate.(*) P < 0.05, (**) P < 0.01 and (***) P < 0.001 versus 0 h