Fig. 4

EPA attenuated iNOS via MyD88/MAP kinase pathway, cells were induced with LPS (1 μg ml^− 1) for 24 h in the presence of EPA. Western blots detecting iNOS (a), MyD88 (b) NFκB (c), (d) p-P38, (e) p-JNK and (f) p-ERK was performed as described in the methodology. Data are shown as mean ± SEM. Statistical differences between groups were assessed by one-way analysis of variance (ANOVA) from at least three independent experiments, followed by Dunnett’s test. * indicates p < 0.05 as compared to LPS and # indicates p < 0.05 as compared to DEX