Fig. 2

N. vomica accelerates motor function recovery after nerve injury. a Time course for muscle grip strength in the mice fed on normal chow (blue circles, n=7) or N. vomica chow (red squares, n=7). The mice were fed on N. vomica chow diet at the time of sciatic nerve crush (dotted line at day 0) and during the whole period of function recovery. Measurements were obtained from both contralateral (dotted lines) and ipsilateral (solid lines). Grip strength is expressed as a percentage of initial force developed at days -8 and -6 (before injury) per individual. Two-way repeated-measure ANOVA (diet x time) showed significant effects of diet (F_(1,13)=6.63, P=0.023) and time (F_(7,91)=63.42, P<0.0001), and a significant interaction between factors (F_(7,91)=4.17, P=0.0005). Post-hoc pairwise comparisons with Benjamini-Hochberg correction revealed significant differences between normal and N. vomica at 4, 6 and 9 days after lesion (*P<0.05; ***P<0.001). b Time course of the sciatic functional index (SFI) of mice as in a. Two-way repeated-measure ANOVA (diet x time) showed a significant effect of time. Post-hoc pairwise comparisons with Benjamini-Hochberg correction revealed significant differences between normal and N. vomica chow at 9 days after lesion (***P<0.001). Gastrocnemius (c) and Tibialis (d) muscle mass in mice as in A. Measurements were obtained after sciatic nerve crush and subsequent functional recovery, and they are expressed as a ratio between ipsilateral and contralateral. Unpaired t-test showed a significant effect of diet on both muscles (**P<0.01; ***P<0.001)