Fig. 2

Effects of HM on cell cycle progression in THP-1 (A) and HEK293 (B) cells. Representative histograms (generated using ModFit LT™ version 5.0.9) of the distribution of cells in the cell cycle, based on DNA staining using propidium iodide (PI) and fluorescence-activated cell sorting (FACS) analysis, after 24 h of incubation of untreated cells (THP-1, A.1; HEK293, B.1) or cells treated with 500 μg/mL HM (THP-1, A.2; HEK293, B.2). Bar graphs present the percentage of cells at each stage of the cell cycle after 24 h of incubation of THP-1 (A.3) and HEK293 (B.3) cells in the presence or absence of various concentrations of HM. Results are presented as means based on three independent experiments