Fig. 5

PCA Scores plot of urinary 1H NMR spectra regarding metabolite concentration (a), (b) and (c). The colors reveal the different analysis periods (CP – blue, AP – red, TP – green). Numbers represent the days of each analysis. (CP – Control period, AP – Anxiogenic Period and TP – treatment period). a Profiles of 3 analyzed periods. The best adjustment and predictability for these models were evaluated by R2 = 0.71 and Q2 = 0.89). b Profiles of AP and TP on days of food restriction. The best adjustment and predictability for these models were evaluated by R2 = 0.76 and Q2 = 0.85). c Profiles of AP and TP on days of ad libitum feeding. The best adjustment and predictability for these models were evaluated by R2 = 0.62 and Q2 = 0.83). d Loadings plot of urinary metabolites responsible for the differentiation of the 3 analyzed periods (CP, AP and TP)